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 | Acceso al texto completo restringido a Biblioteca INIA La Estanzuela. Por información adicional contacte bib_le@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela. |
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Fecha : |
06/12/2019 |
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Actualizado : |
20/04/2020 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
MAYA, L.; MACÍAS-RIOSECO, M.; SILVEIRA, C.S.; GIANNITTI, F.; CASTELLS, M.; RIVERO, R.; CRISTINA, J.; GIANNEECHINI, E.; PUENTES, R.; FLORES, E.F.; RIET-CORREA, F.; COLINA, R. |
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Afiliación : |
Laboratorio de Virología Molecular, CENUR Litoral Norte Sede Salto, Universidad de la República, Gral. Rivera 1350, 50000 Salto, Uruguay; MELISSA MACÍAS RIOSECO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; CAROLINE DA SILVA SILVEIRA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; FEDERICO GIANNITTI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; Laboratorio de Virología Molecular, CENUR Litoral Norte Sede Salto, Universidad de la República, Gral. Rivera 1350, 50000 Salto, Uruguay.; DILAVE Miguel C. Rubino, Laboratorio Regional Noroeste, Casilla De Correo, 57037, CP 60000 Paysandú, Uruguay.; Laboratorio de Virología Molecular, Centro de Investigaciones Nucleares, Facultad de Ciencias, Universidad de la República, Igua 4225, 11400 Montevideo, Uruguay; DILAVE Miguel C. Rubino, Laboratorio Regional Noroeste, Casilla De Correo, 57037, CP 60000 Paysandú, Uruguay.; Departamento de Ciencias Microbiológicas, Facultad de Veterinaria, Universidad de la República, Lasplaces 1550, 11500 Montevideo, Uruguay.; Sector de Virología, Universidad Federal de Santa María, Santa Maria, Rio Grande do Sul, Brazil.; FRANKLIN RIET-CORREA AMARAL, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; RODNEY COLINA, Laboratorio de Virología Molecular, CENUR Litoral Norte Sede Salto, Universidad de la República, Gral. Rivera 1350, 50000 Salto, Uruguay. |
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Título : |
An extensive field study reveals the circulation of new genetic variants of subtype 1a of bovine viral diarrhea virus in Uruguay. |
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Fecha de publicación : |
2020 |
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Fuente / Imprenta : |
Archives of Virology, 1 January 2020, Volume 165, Issue 1, Pages 145-156. Doi: https://doi.org/10.1007/s00705-019-04446-z |
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DOI : |
10.1007/s00705-019-04446-z |
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Idioma : |
Inglés |
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Notas : |
Article history:Received: 22 April 2019 / Accepted: 21 September 2019. |
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Contenido : |
Abstract:
Bovine viral diarrhea virus (BVDV) is a major pathogen worldwide, causing signifcant economic losses to the livestock sector. In Uruguay, BVDV seroprevalence at the farm level is >80%. In this work, 2546 serum, blood or tissue samples collected from animals suspected of being afected by BVD between 2015 and 2017 were analyzed by reverse transcription PCR and sequencing. Analysis of the BVDV genomic regions 5?UTR/Npro, Npro and E2 revealed that BVDV-1a, 1i and 2b circulate
in the country, with BVDV-1a being the most prevalent subtype. Population dynamics studies revealed that BVDV-1a has been circulating in our herds since ~1990. This subtype began to spread and evolve, accumulating point mutations at a rate of 3.48 × 10?3 substitutions/site/year, acquiring specifc genetic characteristics that gave rise to two local genetic lineages of BVDV-1a. These lineages are divergent from those circulating worldwide, as well as the vaccine strain currently used
in Uruguay. The most notable diferences between feld and vaccine strains were found in the E2 glycoprotein, suggesting that the amino acid substitutions could result in failure of cross-protection/neutralization after vaccination. This is the frst study that compares Uruguayan BVDV feld and vaccine strains with other BVDV strains from throughout the world. The results obtained in this study will be very useful for developing a suitable immunization program for BVDV in Uruguay by
identifying local feld strains as candidates for vaccine development. MenosAbstract:
Bovine viral diarrhea virus (BVDV) is a major pathogen worldwide, causing signifcant economic losses to the livestock sector. In Uruguay, BVDV seroprevalence at the farm level is >80%. In this work, 2546 serum, blood or tissue samples collected from animals suspected of being afected by BVD between 2015 and 2017 were analyzed by reverse transcription PCR and sequencing. Analysis of the BVDV genomic regions 5?UTR/Npro, Npro and E2 revealed that BVDV-1a, 1i and 2b circulate
in the country, with BVDV-1a being the most prevalent subtype. Population dynamics studies revealed that BVDV-1a has been circulating in our herds since ~1990. This subtype began to spread and evolve, accumulating point mutations at a rate of 3.48 × 10?3 substitutions/site/year, acquiring specifc genetic characteristics that gave rise to two local genetic lineages of BVDV-1a. These lineages are divergent from those circulating worldwide, as well as the vaccine strain currently used
in Uruguay. The most notable diferences between feld and vaccine strains were found in the E2 glycoprotein, suggesting that the amino acid substitutions could result in failure of cross-protection/neutralization after vaccination. This is the frst study that compares Uruguayan BVDV feld and vaccine strains with other BVDV strains from throughout the world. The results obtained in this study will be very useful for developing a suitable immunization program for BVDV in Uruguay by
identifying local feld strains as candida... Presentar Todo |
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Palabras claves : |
BOVINE VIRAL DIARRHEA (BVD); PLATAFORMA SALUD ANINMAL. |
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Thesagro : |
DIARREA. |
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Asunto categoría : |
L73 Enfermedades de los animales |
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Marc : |
LEADER 02595naa a2200313 a 4500
001 1060513
005 2020-04-20
008 2020 bl uuuu u00u1 u #d
024 7 $a10.1007/s00705-019-04446-z$2DOI
100 1 $aMAYA, L.
245 $aAn extensive field study reveals the circulation of new genetic variants of subtype 1a of bovine viral diarrhea virus in Uruguay.$h[electronic resource]
260 $c2020
500 $aArticle history:Received: 22 April 2019 / Accepted: 21 September 2019.
520 $aAbstract: Bovine viral diarrhea virus (BVDV) is a major pathogen worldwide, causing signifcant economic losses to the livestock sector. In Uruguay, BVDV seroprevalence at the farm level is >80%. In this work, 2546 serum, blood or tissue samples collected from animals suspected of being afected by BVD between 2015 and 2017 were analyzed by reverse transcription PCR and sequencing. Analysis of the BVDV genomic regions 5?UTR/Npro, Npro and E2 revealed that BVDV-1a, 1i and 2b circulate in the country, with BVDV-1a being the most prevalent subtype. Population dynamics studies revealed that BVDV-1a has been circulating in our herds since ~1990. This subtype began to spread and evolve, accumulating point mutations at a rate of 3.48 × 10?3 substitutions/site/year, acquiring specifc genetic characteristics that gave rise to two local genetic lineages of BVDV-1a. These lineages are divergent from those circulating worldwide, as well as the vaccine strain currently used in Uruguay. The most notable diferences between feld and vaccine strains were found in the E2 glycoprotein, suggesting that the amino acid substitutions could result in failure of cross-protection/neutralization after vaccination. This is the frst study that compares Uruguayan BVDV feld and vaccine strains with other BVDV strains from throughout the world. The results obtained in this study will be very useful for developing a suitable immunization program for BVDV in Uruguay by identifying local feld strains as candidates for vaccine development.
650 $aDIARREA
653 $aBOVINE VIRAL DIARRHEA (BVD)
653 $aPLATAFORMA SALUD ANINMAL
700 1 $aMACÍAS-RIOSECO, M.
700 1 $aSILVEIRA, C.S.
700 1 $aGIANNITTI, F.
700 1 $aCASTELLS, M.
700 1 $aRIVERO, R.
700 1 $aCRISTINA, J.
700 1 $aGIANNEECHINI, E.
700 1 $aPUENTES, R.
700 1 $aFLORES, E.F.
700 1 $aRIET-CORREA, F.
700 1 $aCOLINA, R.
773 $tArchives of Virology, 1 January 2020, Volume 165, Issue 1, Pages 145-156. Doi: https://doi.org/10.1007/s00705-019-04446-z
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| 64. |  | ARBOLEYA, J.; FALERO, M. Control de malezas en el cultivo del chícharo. Jornada de divulgación, INIA Las Brujas, 21 noviembre 2013. In: INIA Las Brujas, Programa Nacional Producción Hortícola. Cultivo del chícharo. Jornada de divulgación. Canelones (UY): INIA, 2013. p. 7-13 (INIA Serie Actividades de Difusión; 726)| Biblioteca(s): INIA Las Brujas. |
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| 72. | | ARBOLEYA, J.; FALERO, M.; ACOSTA, P.; RODRÍGUEZ, J. Control de malezas en el cultivo de chícharo ln: DIA DE CAMPO PLAN DE NEGOCIOS DE CHÍCHARO. INIA LAS BRUJAS, 30 DE NOV. DE 2012. PROGRAMA NACIONAL DE INVESTIGACIÓN HORTÍCOLA; FAGRO, DIGEGRA; SFR SANTA ROSA p.6-14 (INIA Serie Actividades de Difusión; 701) "INIA Las Brujas, Programa Nacional de Investigación en Producción Hortícola); Facultad de Agronomía (UdelaR); DIGEGRA-MGAP; SFR Santa Rosa"| Tipo: Actividades de Difusión |
| Biblioteca(s): INIA Las Brujas. |
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| 73. | | ARBOLEYA, J.; FALERO, M.; ACOSTA, P.; RODRÍGUEZ, J. Experimento de control de malezas en INIA Las Brujas ln: DIA DE CAMPO PLAN DE NEGOCIOS DE CHÍCHARO. INIA LAS BRUJAS, 30 DE NOV. DE 2012. PROGRAMA NACIONAL DE INVESTIGACIÓN HORTÍCOLA; FAGRO, DIGEGRA; SFR SANTA ROSA p.22-26 (INIA Serie Actividades de Difusión; 701) "INIA Las Brujas, Programa Nacional de Investigación en Producción Hortícola); Facultad de Agronomía (UdelaR); DIGEGRA-MGAP; SFR Santa Rosa"| Tipo: Actividades de Difusión |
| Biblioteca(s): INIA Las Brujas. |
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| 74. | | ARBOLEYA, J.; FALERO, M.; ACOSTA, P.; RODRÍGUEZ, J. Parcelas de observación de control de malezas en Migues ln: DIA DE CAMPO PLAN DE NEGOCIOS DE CHÍCHARO. INIA LAS BRUJAS, 30 DE NOV. DE 2012. PROGRAMA NACIONAL DE INVESTIGACIÓN HORTÍCOLA; FAGRO, DIGEGRA; SFR SANTA ROSA p.27-29 (INIA Serie Actividades de Difusión; 701) "INIA Las Brujas, Programa Nacional de Investigación en Producción Hortícola); Facultad de Agronomía (UdelaR); DIGEGRA-MGAP; SFR Santa Rosa"| Tipo: Actividades de Difusión |
| Biblioteca(s): INIA Las Brujas. |
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| 75. | | ARBOLEYA, J.; FALERO, M.; GILSANZ, J.; ACOSTA, P. Experimento de control de malezas en Migues. Jornada de divulgación, INIA Las Brujas, 21 noviembre 2013. In: INIA Las Brujas, Programa Nacional Producción Hortícola. Cultivo del chícharo. Jornada de divulgación. Canelones (UY): INIA, 2013. p. 14-19 (INIA Serie Actividades de Difusión; 726) Se agradece al Sr. Raúl Pérez y su familia por su colaboración para llevar adelante este trabajo, como así también a la Ing. Agr. Luján Banchero de DIGEGRA por la coordinación de actividades para la realización de esta actividad.| Biblioteca(s): INIA Las Brujas. |
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| 79. | | ARBOLEYA, J.; GARCIA, C.; SUAREZ, C.; CABOT, M.; GOMEZ, R. Efecto de distintas láminas de agua en dos poblaciones de ajo colorado saneado ln: Día de campo de ajo y cebolla, 1996, Las Brujas, Canelones (UY) Memorias. Las Brujas, Canelones (Uruguay): INIA, 1996. p. 15-17 (INIA Serie Actividades de Difusión ; 117) INIA Las Brujas| Tipo: Actividades de Difusión |
| Biblioteca(s): INIA La Estanzuela; INIA Las Brujas; INIA Tacuarembó. |
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| Registros recuperados : 348 | |
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